iodixanol (optiprep Search Results


iodixanol optipreptm  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc iodixanol optipreptm
Iodixanol Optipreptm, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iodixanol optipreptm/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
iodixanol optipreptm - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
iodixanol (optiprep  (Axis-Shield Diagnostics)
90
Axis-Shield Diagnostics iodixanol (optiprep
Iodixanol (Optiprep, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iodixanol (optiprep/product/Axis-Shield Diagnostics
Average 90 stars, based on 1 article reviews
iodixanol (optiprep - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
iodixanol 40% solution optiprep  (Nycomed)
90
Nycomed iodixanol 40% solution optiprep
Iodixanol 40% Solution Optiprep, supplied by Nycomed, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iodixanol 40% solution optiprep/product/Nycomed
Average 90 stars, based on 1 article reviews
iodixanol 40% solution optiprep - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
discontinuous gradient of iodixanol  (Greiner Bio)
90
Greiner Bio discontinuous gradient of iodixanol
Discontinuous Gradient Of Iodixanol, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/discontinuous gradient of iodixanol/product/Greiner Bio
Average 90 stars, based on 1 article reviews
discontinuous gradient of iodixanol - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
iodixanol was supplied as a 60% w/v solution (optiprep™)  (Axis-Shield Diagnostics)
90
Axis-Shield Diagnostics iodixanol was supplied as a 60% w/v solution (optiprep™)
Iodixanol Was Supplied As A 60% W/V Solution (Optiprep™), supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iodixanol was supplied as a 60% w/v solution (optiprep™)/product/Axis-Shield Diagnostics
Average 90 stars, based on 1 article reviews
iodixanol was supplied as a 60% w/v solution (optiprep™) - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
iodixanol density gradient optiprep  (Greiner Bio)
90
Greiner Bio iodixanol density gradient optiprep
Iodixanol Density Gradient Optiprep, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iodixanol density gradient optiprep/product/Greiner Bio
Average 90 stars, based on 1 article reviews
iodixanol density gradient optiprep - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
iodixanol optiprep  (Serumwerk Bernburg AG)
90
Serumwerk Bernburg AG iodixanol optiprep
Iodixanol Optiprep, supplied by Serumwerk Bernburg AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iodixanol optiprep/product/Serumwerk Bernburg AG
Average 90 stars, based on 1 article reviews
iodixanol optiprep - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
optiprep 60% iodixanol in water  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc optiprep 60% iodixanol in water
Optiprep 60% Iodixanol In Water, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/optiprep 60% iodixanol in water/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
optiprep 60% iodixanol in water - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
iodixanol optiprep-nycomed  (Nycomed)
90
Nycomed iodixanol optiprep-nycomed
Iodixanol Optiprep Nycomed, supplied by Nycomed, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iodixanol optiprep-nycomed/product/Nycomed
Average 90 stars, based on 1 article reviews
iodixanol optiprep-nycomed - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
iodixanol optiprep  (Progen Biotechnik)
90
Progen Biotechnik iodixanol optiprep
Alphaherpesvirus gB localizes to EVs-enriched fractions isolated from virus-infected MJS cells and BoHV-1-infected MDBK. OptiPrep gradient-purification of EVs-enriched and virion-enriched fractions from HSV-1-infected MJS ( A ), BoHV-1-infected MJS ( B ), PRV-infected MJS ( C ), and BoHV-1-infected MDBK ( D ) supernatants. MJS cells were infected with HSV-1 at a multiplicity of infection (moi) of 0.1, with BoHV-1/PRV at an moi of 0.5; MDBK cells were infected at an moi of 0.1. After 50 h post-infection, 0.5 mL fractions were collected from 6%–18% <t>iodixanol</t> (Optiprep) gradient by ultracentrifugation. The 40 µL samples were analyzed in denaturing (for viral glycoprotein C-gC, glycoprotein B-gB, glycoprotein D-gD, for HSV-1, glycoprotein E-gE, for BoHV-1 and PRV or EVs marker Alix) or non-denaturing (for EVs markers CD63 and CD9) SDS-PAGE, and immunoblotted with specific mAbs or polyclonal anti-PRV gB serum. Uninfected (Ø) or virus-infected cell lysates (cl) were analyzed as controls. Bottom panels represent virus titres in each fraction measured by plaque assay. ( E ) Proteins from the indicated fractions (40 μL) were separated by SDS-PAGE, and immunoblotted with antibodies specific to the ICP5/VCP5 homolog of HSV-1 and BoHV-1, virulence factor γ34.5 from HSV-1, immunomodulatory UL49.5 protein of BoHV-1 and envelope glycoproteins gD, and gM, as indicated. HLA-DRβ was detected in the fractions as an EVs marker. Cell lysates (cl) and virus-infected cell lysates (30 μg) were analyzed as controls. ( F ) Comparison of HLA-DRβ levels between EVs from virus-infected and uninfected MJS. DRβ and flotillin-2 (flot-2, EVs marker) from F11 fractions were immunoblotted with specific mAb. Numbers indicate the optical density of DRβ bands from virus-infected MJS EVs, normalized to flot-2 from the same samples, relative to DRβ in EVs from uninfected MJS (set as 1).
Iodixanol Optiprep, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iodixanol optiprep/product/Progen Biotechnik
Average 90 stars, based on 1 article reviews
iodixanol optiprep - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
iodixanol  (Abbott Laboratories)
90
Abbott Laboratories iodixanol
Alphaherpesvirus gB localizes to EVs-enriched fractions isolated from virus-infected MJS cells and BoHV-1-infected MDBK. OptiPrep gradient-purification of EVs-enriched and virion-enriched fractions from HSV-1-infected MJS ( A ), BoHV-1-infected MJS ( B ), PRV-infected MJS ( C ), and BoHV-1-infected MDBK ( D ) supernatants. MJS cells were infected with HSV-1 at a multiplicity of infection (moi) of 0.1, with BoHV-1/PRV at an moi of 0.5; MDBK cells were infected at an moi of 0.1. After 50 h post-infection, 0.5 mL fractions were collected from 6%–18% <t>iodixanol</t> (Optiprep) gradient by ultracentrifugation. The 40 µL samples were analyzed in denaturing (for viral glycoprotein C-gC, glycoprotein B-gB, glycoprotein D-gD, for HSV-1, glycoprotein E-gE, for BoHV-1 and PRV or EVs marker Alix) or non-denaturing (for EVs markers CD63 and CD9) SDS-PAGE, and immunoblotted with specific mAbs or polyclonal anti-PRV gB serum. Uninfected (Ø) or virus-infected cell lysates (cl) were analyzed as controls. Bottom panels represent virus titres in each fraction measured by plaque assay. ( E ) Proteins from the indicated fractions (40 μL) were separated by SDS-PAGE, and immunoblotted with antibodies specific to the ICP5/VCP5 homolog of HSV-1 and BoHV-1, virulence factor γ34.5 from HSV-1, immunomodulatory UL49.5 protein of BoHV-1 and envelope glycoproteins gD, and gM, as indicated. HLA-DRβ was detected in the fractions as an EVs marker. Cell lysates (cl) and virus-infected cell lysates (30 μg) were analyzed as controls. ( F ) Comparison of HLA-DRβ levels between EVs from virus-infected and uninfected MJS. DRβ and flotillin-2 (flot-2, EVs marker) from F11 fractions were immunoblotted with specific mAb. Numbers indicate the optical density of DRβ bands from virus-infected MJS EVs, normalized to flot-2 from the same samples, relative to DRβ in EVs from uninfected MJS (set as 1).
Iodixanol, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iodixanol/product/Abbott Laboratories
Average 90 stars, based on 1 article reviews
iodixanol - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
optiprep 60% w/v aqueous iodixanol  (Cosmo Bio USA)
90
Cosmo Bio USA optiprep 60% w/v aqueous iodixanol
Alphaherpesvirus gB localizes to EVs-enriched fractions isolated from virus-infected MJS cells and BoHV-1-infected MDBK. OptiPrep gradient-purification of EVs-enriched and virion-enriched fractions from HSV-1-infected MJS ( A ), BoHV-1-infected MJS ( B ), PRV-infected MJS ( C ), and BoHV-1-infected MDBK ( D ) supernatants. MJS cells were infected with HSV-1 at a multiplicity of infection (moi) of 0.1, with BoHV-1/PRV at an moi of 0.5; MDBK cells were infected at an moi of 0.1. After 50 h post-infection, 0.5 mL fractions were collected from 6%–18% <t>iodixanol</t> (Optiprep) gradient by ultracentrifugation. The 40 µL samples were analyzed in denaturing (for viral glycoprotein C-gC, glycoprotein B-gB, glycoprotein D-gD, for HSV-1, glycoprotein E-gE, for BoHV-1 and PRV or EVs marker Alix) or non-denaturing (for EVs markers CD63 and CD9) SDS-PAGE, and immunoblotted with specific mAbs or polyclonal anti-PRV gB serum. Uninfected (Ø) or virus-infected cell lysates (cl) were analyzed as controls. Bottom panels represent virus titres in each fraction measured by plaque assay. ( E ) Proteins from the indicated fractions (40 μL) were separated by SDS-PAGE, and immunoblotted with antibodies specific to the ICP5/VCP5 homolog of HSV-1 and BoHV-1, virulence factor γ34.5 from HSV-1, immunomodulatory UL49.5 protein of BoHV-1 and envelope glycoproteins gD, and gM, as indicated. HLA-DRβ was detected in the fractions as an EVs marker. Cell lysates (cl) and virus-infected cell lysates (30 μg) were analyzed as controls. ( F ) Comparison of HLA-DRβ levels between EVs from virus-infected and uninfected MJS. DRβ and flotillin-2 (flot-2, EVs marker) from F11 fractions were immunoblotted with specific mAb. Numbers indicate the optical density of DRβ bands from virus-infected MJS EVs, normalized to flot-2 from the same samples, relative to DRβ in EVs from uninfected MJS (set as 1).
Optiprep 60% W/V Aqueous Iodixanol, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/optiprep 60% w/v aqueous iodixanol/product/Cosmo Bio USA
Average 90 stars, based on 1 article reviews
optiprep 60% w/v aqueous iodixanol - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Alphaherpesvirus gB localizes to EVs-enriched fractions isolated from virus-infected MJS cells and BoHV-1-infected MDBK. OptiPrep gradient-purification of EVs-enriched and virion-enriched fractions from HSV-1-infected MJS ( A ), BoHV-1-infected MJS ( B ), PRV-infected MJS ( C ), and BoHV-1-infected MDBK ( D ) supernatants. MJS cells were infected with HSV-1 at a multiplicity of infection (moi) of 0.1, with BoHV-1/PRV at an moi of 0.5; MDBK cells were infected at an moi of 0.1. After 50 h post-infection, 0.5 mL fractions were collected from 6%–18% iodixanol (Optiprep) gradient by ultracentrifugation. The 40 µL samples were analyzed in denaturing (for viral glycoprotein C-gC, glycoprotein B-gB, glycoprotein D-gD, for HSV-1, glycoprotein E-gE, for BoHV-1 and PRV or EVs marker Alix) or non-denaturing (for EVs markers CD63 and CD9) SDS-PAGE, and immunoblotted with specific mAbs or polyclonal anti-PRV gB serum. Uninfected (Ø) or virus-infected cell lysates (cl) were analyzed as controls. Bottom panels represent virus titres in each fraction measured by plaque assay. ( E ) Proteins from the indicated fractions (40 μL) were separated by SDS-PAGE, and immunoblotted with antibodies specific to the ICP5/VCP5 homolog of HSV-1 and BoHV-1, virulence factor γ34.5 from HSV-1, immunomodulatory UL49.5 protein of BoHV-1 and envelope glycoproteins gD, and gM, as indicated. HLA-DRβ was detected in the fractions as an EVs marker. Cell lysates (cl) and virus-infected cell lysates (30 μg) were analyzed as controls. ( F ) Comparison of HLA-DRβ levels between EVs from virus-infected and uninfected MJS. DRβ and flotillin-2 (flot-2, EVs marker) from F11 fractions were immunoblotted with specific mAb. Numbers indicate the optical density of DRβ bands from virus-infected MJS EVs, normalized to flot-2 from the same samples, relative to DRβ in EVs from uninfected MJS (set as 1).

Journal: Viruses

Article Title: Alphaherpesvirus gB Homologs Are Targeted to Extracellular Vesicles, but They Differentially Affect MHC Class II Molecules

doi: 10.3390/v12040429

Figure Lengend Snippet: Alphaherpesvirus gB localizes to EVs-enriched fractions isolated from virus-infected MJS cells and BoHV-1-infected MDBK. OptiPrep gradient-purification of EVs-enriched and virion-enriched fractions from HSV-1-infected MJS ( A ), BoHV-1-infected MJS ( B ), PRV-infected MJS ( C ), and BoHV-1-infected MDBK ( D ) supernatants. MJS cells were infected with HSV-1 at a multiplicity of infection (moi) of 0.1, with BoHV-1/PRV at an moi of 0.5; MDBK cells were infected at an moi of 0.1. After 50 h post-infection, 0.5 mL fractions were collected from 6%–18% iodixanol (Optiprep) gradient by ultracentrifugation. The 40 µL samples were analyzed in denaturing (for viral glycoprotein C-gC, glycoprotein B-gB, glycoprotein D-gD, for HSV-1, glycoprotein E-gE, for BoHV-1 and PRV or EVs marker Alix) or non-denaturing (for EVs markers CD63 and CD9) SDS-PAGE, and immunoblotted with specific mAbs or polyclonal anti-PRV gB serum. Uninfected (Ø) or virus-infected cell lysates (cl) were analyzed as controls. Bottom panels represent virus titres in each fraction measured by plaque assay. ( E ) Proteins from the indicated fractions (40 μL) were separated by SDS-PAGE, and immunoblotted with antibodies specific to the ICP5/VCP5 homolog of HSV-1 and BoHV-1, virulence factor γ34.5 from HSV-1, immunomodulatory UL49.5 protein of BoHV-1 and envelope glycoproteins gD, and gM, as indicated. HLA-DRβ was detected in the fractions as an EVs marker. Cell lysates (cl) and virus-infected cell lysates (30 μg) were analyzed as controls. ( F ) Comparison of HLA-DRβ levels between EVs from virus-infected and uninfected MJS. DRβ and flotillin-2 (flot-2, EVs marker) from F11 fractions were immunoblotted with specific mAb. Numbers indicate the optical density of DRβ bands from virus-infected MJS EVs, normalized to flot-2 from the same samples, relative to DRβ in EVs from uninfected MJS (set as 1).

Article Snippet: The supernatant was diluted with 60% ( w / v ) iodixanol (OptiPrep, Progen Biotechnik, Heidelberg, Germany) to obtain 6%, and loaded on top of ioxidanol-based gradient ranging from 6 to 18%, with a 1.2% increment (11 mL in volume).

Techniques: Isolation, Virus, Infection, Purification, Marker, SDS Page, Plaque Assay, Comparison